肝癌转移复发模型的建立及其在实验性干预中的应用

本所建立了不同转移潜能的人肝癌裸鼠模型及肝癌细胞株.利用高转移人肝癌裸鼠模型发现大剂量长疗程应用α-干扰素可以抑制肝癌的生长和转移复发,其主要机制可能是通过阻断肿瘤血管生成而发挥作用.     

磁县食管癌的防治

本文总结河北省磁县自1969～2000年食管癌的死亡情况及防治结果.磁县自20世纪60年代至21世纪初,由于持续采取综合防治措施,食管癌发病率明显下降,各年代依次为140.88/10万、128.35/10万、107.95/万、89.10/10万、86.17/10万,趋势检验χ2=563.40,P＜0.001,有显著差异.     

Chronic consumption of fructose in combination with <Emphasis Type="Italic">trans</Emphasis> fatty acids but not with saturated fatty acids induces nonalcoholic steatohepatitis with fibrosis in rats

Purpose

Consumption of Western diet high in fat and fructose has been attributed to the recent epidemic of nonalcoholic fatty liver disease (NAFLD). However, the impact of specific fatty acids on the progression of NAFLD to nonalcoholic steatohepatitis (NASH) is poorly understood. In the present study, we investigated the chronic effects of consumption of fructose in combination with saturated fatty acids (SFA) or trans fatty acids (TFA) on the development of NAFLD.

Methods

Male Sprague–Dawley rats were randomly assigned to six isocaloric starch/high fructose (44% of calories), high fat (39% calories) diet containing either starch–peanut oil, fructose–peanut oil, fructose–palmolein, fructose–clarified butter, fructose–coconut oil or fructose–partially hydrogenated vegetable oil and fed for 24 weeks. Palmolein, clarified butter and coconut oil were used as the source of SFA whereas partially hydrogenated vegetable oil was used as the source of TFA. Peanut oil was used as the reference oil.

Results

Long-term feeding of fructose in combination with SFA or TFA induced hepatic steatosis of similar extent associated with upregulation of stearoyl CoA desaturase-1. In contrast, fructose in combination with TFA induced NASH with fibrosis as evidenced by upregulation of hepatic proinflammatory cytokine and fibrogenic gene expression, increased hepatic oxidative stress and adipocytokine imbalance. Histopathological analysis revealed the presence of NASH with fibrosis. Further, peanut oil prevented the development of NAFLD in fructose-fed rats.

Conclusion

Fructose in combination with TFA caused NASH with fibrosis by inducing oxidative stress and inflammation, whereas, fructose in combination with SFA caused simple steatosis, suggesting that the type of fatty acid is more important for the progression of NAFLD.

     

降低咬合对牙髓治疗后疼痛的影响

目的：探讨降低咬合对牙髓治疗后疼痛的影响。方法：将患牙105颗分为全面降低咬合组（1组）、模拟降低咬合组(2组)和对照组（3组），分析它们在有无牙髓活力、根周X线透射区、叩痛或术前疼痛等情形下的术后疼痛反应。结果：对于活髓、无根周X线透射区、有叩痛、有术前疼痛的患牙，1组与2组、3组之间的疼痛发生率有显著差异，而其余相反4种情形下，3组之间的疼痛发生率无显著差异。结论：降低咬合有助于减轻牙髓治疗术后疼痛。     

非负荷期种植体周围牙槽骨丧失的X线观察

目的：研究非负荷期种植体周围骨吸收的时间变化规律。方法：以非埋植型纯钛种植体植入狗下颌骨，术后每月拍一次标准牙片，用Photoshop 6.01软件测量种植体周围骨吸收的量并加以分析。结果：种植体植入后第一个月骨吸收为0．64mm，每二个月为0．01mm，第三、四、五个月分别为0．04mm,0.15mm,0.24mm，第一个月骨吸收量与其余四个月之间有显著性差异（P＜0．05）。结论：种植体植入后第一个月骨吸收最多，可能与手术创伤引起的骨组织改建局部加速现象有关。     

DF暂封糊剂的研制

目的：研制一种单组份、糊剂型、水硬性暂封材料。方法：通过测定材料在水中的凝固速度及凝固后材料表面性状，对水硬性材料成份的种类、赋形剂的种类及凝固促进剂的种类进行筛选。结果：水硬性材料Ａ２的凝固速度最快，赋形剂Ｂ２的效果最好，凝固促进剂Ｃ１的作用优于Ｃ２。结论：采用Ａ２、Ｂ２、Ｃ１作为暂封材料基本组成效果最佳，凝固２４ｈ后抗压强度达１３．９４ＭＰａ±２．０７ＭＰａ。     

上颌第一前磨牙髓腔形态的影像学研究

目的:利用RVG(radio visio graphy)数字化X线成像系统测量上颌第一前磨牙髓腔解剖形态的相关数值,为临床诊断、治疗提供重要参考依据。方法:收集拔除的上颌第一前磨牙70颗,牙齿发育完全、根尖完整,用RVG数字成像系统分别拍摄近远中向数字X线片,在图像上测量髓腔形态的相关数值。结果:上颌第一前磨牙颈部髓室的颊腭向厚度(4.34±0.49)mm,颊尖至颊侧髓角之间的距离(5.84±0.58)mm,腭尖至腭侧髓角之间的距离(5.95±0.56)mm;牙尖至髓顶的距离(7.62±0.75)mm,牙尖至髓底的距离(10.38±1.44)mm;髓室高度(2.98±0.98)mm;髓顶至根分叉的距离(7.67±1.79)mm,髓底至根分叉的距离(4.59±1.48)mm。2根管口之间的距离(3.22±0.76)mm,2根管根中1/2之间的距离(3.71±1.43)mm,2根尖孔之间的距离(3.91±2.23)mm。结论:提供成人上颌第一前磨牙髓腔解剖的形态特征,为临床诊断和治疗时参考。